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Atomic Force Microscopy

Atomic Force Microscopy

Author: Pier Carlo Braga, Davide Ricci
Publisher: Springer Science & Business Media
ISBN: 1592596479
Pages: 394
Year: 2004
Highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.
Atomic Force Microscopy in Cell Biology

Atomic Force Microscopy in Cell Biology

Author:
Publisher: Academic Press
ISBN: 0080549446
Pages: 415
Year: 2002-05-30
This is the first book to cover the history, structure, and application of atomic force microscopy in cell biology. Presented in the clear, well-illustrated style of the Methods in Cell Biology series, it introduces the AFM to its readers and enables them to tap the power and scope of this technology to further their own research. A practical laboratory guide for use of the atomic force and photonic force microscopes, it provides updated technology and methods in force spectroscopy. It is also a comprehensive and easy-to-follow practical laboratory guide for the use of the AFM and PFM in biological research.
Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology

Author: Keith Wilson, John Walker
Publisher: Cambridge University Press
ISBN: 0521828899
Pages: 783
Year: 2005-03-21
This is a new and expanded edition of the bestselling Principles and Techniques of Practical Biochemistry. It includes discussion of the theoretical principles, practical details and applications of the key experimental techniques that are routinely used in modern biochemistry and molecular biology, including those that have led to the emergence of the new disciplines of genomics, proteomics and bioinformatics. All chapters have been updated and new sections added to cover the principles of experimental design, the statistical analysis of quantitative analytical data, and the principles and practice of clinical biochemistry. here are new chapters covering cell culture, microscopy and mass spectrometry. To enhance student understanding of each topic, in-text worked examples are included in each chapter. The textbook will be essential reading for all bioscience students and pre-clinical medical students.
Atomic Force Microscopy in Biomedical Research

Atomic Force Microscopy in Biomedical Research

Author: Pier Carlo Braga, Davide Ricci
Publisher: Humana Press
ISBN: 1617791067
Pages: 508
Year: 2011-08-25
With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Scanning Probe Microscopy

Scanning Probe Microscopy

Author: Sergei V. Kalinin, Alexei Gruverman
Publisher: Springer Science & Business Media
ISBN: 0387286683
Pages: 980
Year: 2007-04-03
This volume will be devoted to the technical aspects of electrical and electromechanical SPM probes and SPM imaging on the limits of resolution, thus providing technical introduction into the field. This volume will also address the fundamental physical phenomena underpinning the imaging mechanism of SPMs.
Nano-Bio-Sensing

Nano-Bio-Sensing

Author: Sandro Carrara
Publisher: Springer Science & Business Media
ISBN: 1441961690
Pages: 248
Year: 2010-11-01
The application of circuits and systems and engineering principles to problems in the medicine has led to the emergence of biomedical circuits and systems as an exciting and rapidly growing area of research. Nanotechnology provides new nano-structured materials with amazing properties. The properties offered by nanomaterials can be applied to develop advanced instrumentation for biomedical diagnostics and personalized therapy, as well as bio-sensing in the environment. Biotechnology provides new biochemical materials with novel properties to be applied to develop new performances in sensing techniques. These advancements in Nano- and Bio- technologies will lead to new concepts and applications for nano-bio-sensing systems. This book offers an invaluable reference to the state-of-the-art applications of nano-bio-sensing. It brings together expertise of researchers from the fields of nano-electronics and bio-technology, providing multidisciplinary content from nano-structures fabrication to bio-sensing applications.
Bioimaging: Current Concepts in Light & Electron Microscopy

Bioimaging: Current Concepts in Light & Electron Microscopy

Author: Douglas E Chandler, Robert W. Roberson
Publisher: Jones & Bartlett Publishers
ISBN: 0763738743
Pages: 440
Year: 2009
The development of microscopy revolutionized the world of cell and molecular biology as we once knew it and will continue to play an important role in future discoveries. Bioimaging: Current Concepts in Light and Electron Microscopy is the optimal text for any undergraduate or graduate bioimaging course, and will serve as an important reference tool for the research scientist. This unique text covers, in great depth, both light and electron microscopy, as well as other structure and imaging techniques like x-ray crystallography and atomic force microscopy. Written in a user-friendly style and covering a broad range of topics, Bioimaging describes the state-of-the-art technologies that have powered the field to the forefront of cellular and molecular biological research. Important Notice: The digital edition of this book is missing some of the images or content found in the physical edition.
Progress in Adhesion and Adhesives

Progress in Adhesion and Adhesives

Author: K. L. Mittal
Publisher: John Wiley & Sons
ISBN: 1119162335
Pages: 512
Year: 2015-07-27
This book is based on the 13 review articles written by subject experts and published in 2014 in the Journal Reviews of Adhesion and Adhesives. The rationale for publication of this book is that currently the RAA has limited circulation, so this book provides broad exposure and dissemination of the concise, critical, illuminating, and thought-provoking review articles. The subjects of the reviews fall into 4 general areas: 1. Polymer surface modification 2. Biomedical, pharmaceutical and dental fields 3. Adhesives and adhesive joints 4. General Adhesion Aspects The topics covered include: Adhesion of condensed bodies at microscale; imparting adhesion property to silicone material; functionally graded adhesively bonded joints; synthetic adhesives for wood panels; adhesion theories in wood adhesive bonding; adhesion and surface issues in biocomposites and bionanocomposites; adhesion phenomena in pharmaceutical products and applications of AFM; cyanoacrylate adhesives in surgical applications; ways to generate monosort functionalized polyolefin surfaces; nano-enhanced adhesives; bonding dissimilar materials in dentistry; flame treatment of polymeric materials—relevance to adhesion; and mucoadhesive polymers for enhancing retention of ocular drug delivery.
Atomic Force Microscopy in Biomedical Research

Atomic Force Microscopy in Biomedical Research

Author: Pier Carlo Braga, Davide Ricci
Publisher: Humana Press
ISBN: 1617791040
Pages: 508
Year: 2011-05-20
With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Techniques in Microscopy for Biomedical Applications

Techniques in Microscopy for Biomedical Applications

Author: Terje Dokland
Publisher: World Scientific
ISBN: 9812564349
Pages: 378
Year: 2006
The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.
Cell Imaging Techniques

Cell Imaging Techniques

Author: Douglas J. Taatjes, Brooke T. Mossman
Publisher: Springer Science & Business Media
ISBN: 1592599931
Pages: 490
Year: 2006
Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.
Global Perspectives of Nanoscience and Engineering Education

Global Perspectives of Nanoscience and Engineering Education

Author: Kurt Winkelmann, Bharat Bhushan
Publisher: Springer
ISBN: 3319318330
Pages: 400
Year: 2016-06-28
This book presents the perspectives of nanotechnology educators from around the world. Experts present the pressing challenges of teaching nanoscience and engineering to students in all levels of education, postsecondary and informal environments. The book was inspired by the 2014 NSF workshop for Nanoscience and Engineering Education. Since nanotechnology is a relatively new field, authors present recommendations for designing nanotechnology education programs. The chapters describe methods to teach specific topics, such as probe microscopy, size and scale, and nanomaterial safety, in classrooms around the world. Other chapters describe the ways that organizations like NNIN and the NISE Network have influenced informal nanotechnology education. Information technology plays a growing role in all types of education and several chapters are devoted to describing ways how educators can use online curricula for teaching nanotechnology to students from preschool to graduate school.
Protein-ligand Interactions

Protein-ligand Interactions

Author: Gerd Ulrich Nienhaus
Publisher: Springer
ISBN: 1592599125
Pages: 568
Year: 2005-01-01
A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Advance of Polymers Applied to Biomedical Applications: Cell Scaffolds

Advance of Polymers Applied to Biomedical Applications: Cell Scaffolds

Author: Insung S. Choi , João F. Mano
Publisher: MDPI
ISBN: 3038970336
Pages: 406
Year: 2018-09-04
This book is a printed edition of the Special Issue "Advance of Polymers Applied to Biomedical Applications: Cell Scaffolds" that was published in Polymers
Chemical Biology

Chemical Biology

Author: Banafshe Larijani, Colin A. Rosser, Rudiger Woscholski
Publisher: John Wiley & Sons
ISBN: 0470090669
Pages: 272
Year: 2006-08-04
Written by a team of international researchers and teachers at the cutting edge of chemical biology research, this book provides an exciting, comprehensive introduction to a wide range of chemical and physical techniques with applications in areas as diverse as molecular biology, signal transduction, drug discovery and medicine. Techniques include: Cryo-electron microscopy, atomic force microscopy, differential scanning calorimetry in the study of lipid structures, membrane potentials and membrane probes, identification and quantification of lipids using mass spectroscopy, liquid state NMR, solid state NMR in biomembranes, molecular dynamics, two dimensional infra-red studies of biomolecules, single and two-photon fluorescence, optical tweezers, PET imaging and chemical genetics. KEY FEATURES: a unique guide to the rapidly evolving, interdisciplinary field of chemical biology. adopts a molecular structure for maximum flexibility. addresses relevant, topical chemical biological questions throughout. includes stunning illustrations. associates website with PowerPoint slides of figures within the book. Chemical Biology: Techniques and Applications provides an invaluable resource for final year undergraduate and post graduate bioscience and biomedical students and pharmaceutical researchers with an interest in this fascinating, and ever changing field.